Pglo Restriction Map

Pglo Restriction Map. [Solved] fill out this form Consider the following restriction map of araC P BAD GFP ampR ampR promoter ori NdeI (4,927) ClaI (2) EcoRV (386) This is generally done by breaking done the membrane of the cell, allowing DNA to move into the cell

Use a restriction map to predict how many fragments will be produced in a given restriction digest. Specific sequences, including gene and promoter sequences, are shown as thick lines along the

Solved 2. Based on the analysis of the PGLO digests,

Customize plasmid maps with flexible annotation and visualization controls; Automatically generate a rich graphical history of every edit and procedure Analyze a DNA sequence to see restriction sites and map Specific sequences, including gene and promoter sequences, are shown as thick lines along the

Plasmids 101 Restriction Cloning. Detailed frequencies with which restriction endonuclease sites occur in commonly used DNA molecules can be found on Frequencies of Restriction Sites The plasmid size of pGLO is 5371 bp and the restriction sites for for PstI are at 2106 and 3181

pGlo Biology OER. notes, and annotations in the zip file on this page are copyrighted material Restriction maps are made by first digesting a piece of DNA with a restriction enzyme, then separating the fragments using agarose gel